Using bulk-RNA sequencing to compare transcritomes of cells with senescence-associated heterochromatin foci (SAHF) positive or negative responses after oncogene-induced senesence.

Nucleoli have been associated with longevity in c. elegans and mammalian models and nucleolar size could be used as a biomarker for determining age. At middle-age, there is heterogenity in nucleolar size, even in cells living within the same environment. This project focuses on using in-house methods to separate same-cell populations based on nucleolar size, for future comparison of the cell transcriptome, which could provide an explanation on why some cells develop larger nucleoli sooner than others. Additionally, this project will look at nucleolar-specific marker fibrillarin across cells with larger and smaller nucleoli and in the context of oncogene-induced senescence.