Impact of miRNA modulation on cerebral cells in an in vitro model of hypoxia reoxygenation

University of Glasgow

Past award

Student: Catriona McDonald : University of Glasgow

Year Award Started: 2013

miRNAs have been implicated as key modulators of pathogenesis in numerous diseases. This project aims to assess the role of a specific miRNA, selected from a previously performed openarray, following hypoxic injury in cerebral cells. The study will utilise a neuronal and brain-derived endothelial cell line in an optimised model of in vitro hypoxia / reoxygenation. Using pre-miR’s to increase and antagomiRs to knockdown the endogenous levels of the specific miRNA, the mechanistic action of the miRNA can be assessed using a number of previously optimised assays of oxidative stress, apoptosis and cell proliferation.

Research area: Other conditions


Dr Emily Ord
Institute of Cardiovascular and Medical Sciences